Modularity of select riboswitch expression platforms enables facile engineering of novel genetic regulatory devices.

ABSTRACT: RNA-based biosensors and regulatory devices have received significant attention for their potential in a broad array of synthetic biology applications. One of the primary difficulties in engineering these molecules is the lack of facile methods to link sensory modules, or aptamers, to readout domains. Such efforts typically require extensive screening or selection of sequences that facilitate interdomain communication. Bacteria have evolved a widespread form of gene regulation known as riboswitches that perform this task with sufficient fidelity to control expression of biosynthetic and transport proteins essential for normal cellular homeostasis. In this work, we demonstrate that select riboswitch readout domains, called expression platforms, are modular in that they can host a variety of

SUBMITTER: Ceres P 

PROVIDER: S-EPMC3742664 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature