High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

ABSTRACT: The generation of induced pluripotent stem (iPS) cells presents a challenge to normal developmental processes. The low efficiency and heterogeneity of most methods have hindered understanding of the precise molecular mechanisms promoting, and roadblocks preventing, efficient reprogramming. Although several intermediate populations have been described, it has proved difficult to characterize the rare, asynchronous transition from these intermediate stages to iPS cells. The rapid expansion of minor reprogrammed cells in the heterogeneous population can also obscure investigation of relevant transition processes. Understanding the biological mechanisms essential for successful iPS cell generation requires both accurate capture of cells undergoing the reprogramming process and identification o

SUBMITTER: O'Malley J 

PROVIDER: S-EPMC3743022 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature