Dataset Information

Proteomic serum profile of fatigued men receiving localized external beam radiation therapy for non-metastatic prostate cancer.

ABSTRACT:

Context

Fatigue is the most distressing side effect of radiation therapy, and its progression etiology is unknown.

Objectives

This study describes proteome changes from sera of fatigued men with non-metastatic prostate cancer receiving external beam radiation therapy (EBRT).

Methods

Fatigue scores, measured by the Functional Assessment of Chronic Illness Therapy-Fatigue, and serum were collected from 12 subjects at baseline (before EBRT) and at midpoint (Day 21) of EBRT. Depleted sera from both time points were analyzed using two-dimensional difference gel electrophoresis, and up/down regulated proteins were identified using liquid chromatography-tandem mass spectrometry. Western blot analyses confirmed the protein changes observed.