Development of a model of elevated intraocular pressure in rats by gene transfer of bone morphogenetic protein 2.

ABSTRACT:

Purpose

To determine whether inducing calcification in the trabecular meshwork results in elevated IOP in living rats. To use this property to create an elevated IOP animal model by gene transfer of bone morphogenetic protein 2 (BMP2).

Methods

Calcification was assessed by alizarin red staining in primary human trabecular meshwork (HTM) cells and alkaline phosphatase (ALP) activity in the angle tissue. Brown Norway (BN) and Wistar rats were intracamerally injected with Ad5BMP2 (OS) and control Ad5.CMV-Null (OD). IOPs were taken twice a week and expressed as mean integral pressures. Morphology was assessed on fixed, paraffin-embedded anterior segments. Retinal ganglion cells (RGCs) were quantified on retrograde and Brn-3a-labeled flat mounts using MetaMorph software.

Resul

SUBMITTER: Buie LK 

PROVIDER: S-EPMC3743456 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature