A 50-gene intrinsic subtype classifier for prognosis and prediction of benefit from adjuvant tamoxifen.

ABSTRACT:

Purpose

Gene expression profiling classifies breast cancer into intrinsic subtypes based on the biology of the underlying disease pathways. We have used material from a prospective randomized trial of tamoxifen versus placebo in premenopausal women with primary breast cancer (NCIC CTG MA.12) to evaluate the prognostic and predictive significance of intrinsic subtypes identified by both the PAM50 gene set and by immunohistochemistry.

Experimental design

Total RNA from 398 of 672 (59%) patients was available for intrinsic subtyping with a quantitative reverse transcriptase PCR (qRT-PCR) 50-gene predictor (PAM50) for luminal A, luminal B, HER-2-enriched, and basal-like subtypes. A tissue microarray was also constructed from 492 of 672 (73%) of the study population to assess a p

SUBMITTER: Chia SK 

PROVIDER: S-EPMC3743663 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature