Effective Alu repeat based RT-Qpcr normalization in cancer cell perturbation experiments.

ABSTRACT:

Background

Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories. A specific set of mRNAs as internal control reference genes is considered as the preferred strategy to normalize RT-qPCR data. Proper selection of reference genes is a critical issue, especially in cancer cells that are subjected to different in vitro manipulations. These manipulations may result in dramatic alterations in gene expression levels, even of assumed reference genes. In this study, we evaluated the expression levels of 11 commonly used reference genes as internal controls for normalization of 19 experiments that include neuroblastoma, T-ALL, melanoma, breast cancer, non small cell lung cancer (NSCL), a

SUBMITTER: Rihani A 

PROVIDER: S-EPMC3743747 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature