Visualisation of γH2AX foci caused by heavy ion particle traversal; distinction between core track versus non-track damage.

ABSTRACT: Heavy particle irradiation produces complex DNA double strand breaks (DSBs) which can arise from primary ionisation events within the particle trajectory. Additionally, secondary electrons, termed delta-electrons, which have a range of distributions can create low linear energy transfer (LET) damage within but also distant from the track. DNA damage by delta-electrons distant from the track has not previously been carefully characterised. Using imaging with deconvolution, we show that at 8 hours after exposure to Fe (∼200 keV/µm) ions, γH2AX foci forming at DSBs within the particle track are large and encompass multiple smaller and closely localised foci, which we designate as clustered γH2AX foci. These foci are repaired with slow kinetics by DNA non-homologous end-joining (NHEJ) in G1 ph

SUBMITTER: Nakajima NI 

PROVIDER: S-EPMC3743843 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature