Characterization of the p53 cistrome--DNA binding cooperativity dissects p53's tumor suppressor functions.

ABSTRACT: p53 protects us from cancer by transcriptionally regulating tumor suppressive programs designed to either prevent the development or clonal expansion of malignant cells. How p53 selects target genes in the genome in a context- and tissue-specific manner remains largely obscure. There is growing evidence that the ability of p53 to bind DNA in a cooperative manner prominently influences target gene selection with activation of the apoptosis program being completely dependent on DNA binding cooperativity. Here, we used ChIP-seq to comprehensively profile the cistrome of p53 mutants with reduced or increased cooperativity. The analysis highlighted a particular relevance of cooperativity for extending the p53 cistrome to non-canonical binding sequences characterized by deletions, spacer inserti

SUBMITTER: Schlereth K 

PROVIDER: S-EPMC3744428 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature