Multi-gene detection and identification of mosquito-borne RNA viruses using an oligonucleotide microarray.

ABSTRACT:

Background

Arthropod-borne viruses are important emerging pathogens world-wide. Viruses transmitted by mosquitoes, such as dengue, yellow fever, and Japanese encephalitis viruses, infect hundreds of millions of people and animals each year. Global surveillance of these viruses in mosquito vectors using molecular based assays is critical for prevention and control of the associated diseases. Here, we report an oligonucleotide DNA microarray design, termed ArboChip5.1, for multi-gene detection and identification of mosquito-borne RNA viruses from the genera Flavivirus (family Flaviviridae), Alphavirus (Togaviridae), Orthobunyavirus (Bunyaviridae), and Phlebovirus (Bunyaviridae).

Methodology/principal findings

The assay utilizes targeted PCR amplification of three genes from ea

SUBMITTER: Grubaugh ND 

PROVIDER: S-EPMC3744434 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature