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ABF transcription factors of Thellungiella salsuginea: Structure, expression profiles and interaction with 14-3-3 regulatory proteins.


ABSTRACT: ABF transcription factors are the key regulators of ABA signaling. Using RACE-PCR, we identified and sequenced the coding regions of four genes that encode ABF transcription factors in the extremophile plant Thellungiella salsuginea, a close relative of Arabidopsis thaliana that possesses high tolerance to abiotic stresses. An analysis of the deduced amino acid sequences revealed that the similarity between Thellungiella and Arabidopsis ABFs ranged from 71% to 88%. Similar to their Arabidopsis counterparts, Thellungiella ABFs share a bZIP domain and four conservative domains, including a highly conservative motif at the C-terminal tail, which was reported to be a canonical site for binding by 14-3-3 regulatory proteins. Gene expression analysis by real-time PCR revealed a rapid transcript

SUBMITTER: Vysotskii DA 

PROVIDER: S-EPMC3745569 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature