Alternative strategies for development of a reference transcriptome for quantification of allele specific expression in organisms having sparse genomic resources.

ABSTRACT: In recent years RNA-Seq technology has been used not only to quantify differences in gene expression but also to understand the underlying mechanisms that lead to these differences. Nucleotide sequence variation arising through evolution may differentially affect the expression profiles of divergent species. RNA-Seq technology, combined with techniques to differentiate parental alleles and quantify their abundance, have recently become popular methods for allele specific gene expression (ASGE) analyses. However, analysis of gene expression within interspecies hybrids may be difficult when one of the two parental genomes represented in the hybrid does not have robust genomic resources or available transcriptome data. Herein, we compare two strategies for analyzing allele specific expression

SUBMITTER: Shen Y 

PROVIDER: S-EPMC3746558 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature