Differential transcript isoform usage pre- and post-zygotic genome activation in zebrafish.

ABSTRACT:

Background

Zebrafish embryos are transcriptionally silent until activation of the zygotic genome during the 10th cell cycle. Onset of transcription is followed by cellular and morphological changes involving cell speciation and gastrulation. Previous genome-wide surveys of transcriptional changes only assessed gene expression levels; however, recent studies have shown the necessity to map isoform-specific transcriptional changes. Here, we perform isoform discovery and quantification on transcriptome sequences from before and after zebrafish zygotic genome activation (ZGA).

Results

We identify novel isoforms and isoform switches during ZGA for genes related to cell adhesion, pluripotency and DNA methylation. Isoform switching events include alternative splicing and changes in

SUBMITTER: Aanes H 

PROVIDER: S-EPMC3747860 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature