Transcription factor σB plays an important role in the production of extracellular membrane-derived vesicles in Listeria monocytogenes.

ABSTRACT: Gram-negative bacteria produce extracellular outer membrane vesicles (OMVs) that interact with host cells. Unlike Gram-negative bacteria, less is known about the production and role of extracellular membrane vesicles (MVs) in Gram-positive bacteria. The food-borne pathogen Listeria monocytogenes can survive under extreme environmental and energy stress conditions and the transcription factor σ(B) is involved in this survival ability. Here, we first determined the production of MVs from L. monocytogenes and evaluated whether general stress transcription factor σ(B) affected production of MVs in L. monocytogenes. L. monocytogenes secreted MVs during in vitro broth culture. The wild-type strain actively produced MVs approximately nine times more and also produced more intact shapes of MVs tha

SUBMITTER: Lee JH 

PROVIDER: S-EPMC3748028 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature