Lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENP-E and MCAK.

ABSTRACT:

Background

Proper attachment of chromosomes to microtubules is crucial for the accurate segregation of chromosomes. Human chromosomes attach initially to lateral walls of microtubules. Subsequently, attachments to lateral walls disappear and attachments to microtubule ends (end-on attachments) predominate. While it is known in yeasts that lateral to end-on conversion of attachments occurs through a multistep process, equivalent conversion steps in humans remain unknown.

Results

By developing a high-resolution imaging assay to visualize intermediary steps of the lateral to end-on conversion process, we show that the mechanisms that bring a laterally bound chromosome and its microtubule end closer to each other are indispensable for proper end-on attachment because laterally a

SUBMITTER: Shrestha RL 

PROVIDER: S-EPMC3748344 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature