MicroRNA-128 coordinately targets Polycomb Repressor Complexes in glioma stem cells.

ABSTRACT:

Background

The Polycomb Repressor Complex (PRC) is an epigenetic regulator of transcription whose action is mediated by 2 protein complexes, PRC1 and PRC2. PRC is oncogenic in glioblastoma, where it is involved in cancer stem cell maintenance and radioresistance.

Methods

We used a set of glioblastoma patient samples, glioma stem cells, and neural stem cells from a mouse model of glioblastoma. We characterized gene/protein expression and cellular phenotypes by quantitative PCR/Western blotting and clonogenic, cell-cycle, and DNA damage assays. We performed overexpression/knockdown studies by lentiviral infection and microRNA/small interfering RNA oligonucleotide transfection.

Results

We show that microRNA-128 (miR-128) directly targets mRNA of SUZ12, a key component of

SUBMITTER: Peruzzi P 

PROVIDER: S-EPMC3748913 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature