Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast-rich Culture.

ABSTRACT:

Objectives

This study investigated the feasibility of using a phosphorescence oxygen analyser to measure cellular respiration (mitochondrial O2 consumption) in foreskin samples and their fibroblast-rich cultures.

Methods

Foreskin specimens from normal infants were collected immediately after circumcision and processed for measuring cellular respiration and for culture. Cellular mitochondrial O2 consumption was determined as a function of time from the phosphorescence decay of the Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin.

Results

In sealed vials containing a foreskin specimen and glucose, O2 concentration decreased linearly with time, confirming the zero-order kinetics of O2 consumption by cytochrome oxidase. Cyanide inhibited O2 consumption, confirmi

SUBMITTER: Al-Jasmi F 

PROVIDER: S-EPMC3749026 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature