DUSP6 regulates drug sensitivity by modulating DNA damage response.

ABSTRACT:

Background

Dual specificity phosphatase 6 (DUSP6) is a member of a family of mitogen-activated protein kinase phosphatases that dephosphorylates and inhibits activated ERK1/2. Dual specificity phosphatase 6 is dynamically regulated in developmental and pathological conditions such as cancer.

Methods

Cancer cell lines were made deficient in DUSP6 by siRNA and shRNA silencing. Sensitivity to anti-EGFR and chemotherapeutic agents was determined in viability and apoptosis assays, and in xenografts established in SCID mice. Cellular effects of DUSP6 inactivation were analysed by proteomic methods, followed by analysis of markers of DNA damage response (DDR) and cell cycle.

Results

We determined that depletion of DUSP6 reduced the viability of cancer cell lines and increase

SUBMITTER: Bagnyukova TV 

PROVIDER: S-EPMC3749559 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature