A tandem chemoenzymatic methylation by S-adenosyl-L-methionine.

ABSTRACT: Keep 'em methylated: The in situ preparation of the cofactor AdoMet was achieved by allowing the biosynthetic enzyme SalL to operate in the reverse direction by presentation of 5'-chloro-5'-deoxyadenosine at low salt concentrations. This reaction was readily coupled with DNA and small molecule methyltransferases to afford a regioselective method for chemo-enzymatic methylation and isotope incorporation.

SUBMITTER: Lipson JM 

PROVIDER: S-EPMC3749784 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature