Nucleosome free regions in yeast promoters result from competitive binding of transcription factors that interact with chromatin modifiers.

ABSTRACT: Because DNA packaging in nucleosomes modulates its accessibility to transcription factors (TFs), unraveling the causal determinants of nucleosome positioning is of great importance to understanding gene regulation. Although there is evidence that intrinsic sequence specificity contributes to nucleosome positioning, the extent to which other factors contribute to nucleosome positioning is currently highly debated. Here we obtained both in vivo and in vitro reference maps of positions that are either consistently covered or free of nucleosomes across multiple experimental data-sets in Saccharomyces cerevisiae. We then systematically quantified the contribution of TF binding to nucleosome positioning using a rigorous statistical mechanics model in which TFs compete with nucleosomes for bindin

SUBMITTER: Ozonov EA 

PROVIDER: S-EPMC3749953 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature