Microarray characterization of gene expression changes in blood during acute ethanol exposure.

ABSTRACT:

Background

As part of the civil aviation safety program to define the adverse effects of ethanol on flying performance, we performed a DNA microarray analysis of human whole blood samples from a five-time point study of subjects administered ethanol orally, followed by breathalyzer analysis, to monitor blood alcohol concentration (BAC) to discover significant gene expression changes in response to the ethanol exposure.

Methods

Subjects were administered either orange juice or orange juice with ethanol. Blood samples were taken based on BAC and total RNA was isolated from PaxGene™ blood tubes. The amplified cDNA was used in microarray and quantitative real-time polymerase chain reaction (RT-qPCR) analyses to evaluate differential gene expression. Microarray data was analyzed

SUBMITTER: Kupfer DM 

PROVIDER: S-EPMC3750403 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature