RNA-Seq analysis and targeted mutagenesis for improved free fatty acid production in an engineered cyanobacterium.

ABSTRACT:

Background

High-energy-density biofuels are typically derived from the fatty acid pathway, thus establishing free fatty acids (FFAs) as important fuel precursors. FFA production using photosynthetic microorganisms like cyanobacteria allows for direct conversion of carbon dioxide into fuel precursors. Recent studies investigating cyanobacterial FFA production have demonstrated the potential of this process, yet FFA production was also shown to have negative physiological effects on the cyanobacterial host, ultimately limiting high yields of FFAs.

Results

Cyanobacterial FFA production was shown to generate reactive oxygen species (ROS) and lead to increased cell membrane permeability. To identify genetic targets that may mitigate these toxic effects, RNA-seq analysis was used

SUBMITTER: Ruffing AM 

PROVIDER: S-EPMC3750487 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature