Analysis of the transcriptome of the Indonesian coelacanth Latimeria menadoensis.

ABSTRACT:

Background

Latimeria menadoensis is a coelacanth species first identified in 1997 in Indonesia, at 10,000 Km of distance from its African congener. To date, only six specimens have been caught and just a very limited molecular data is available. In the present work we describe the de novo transcriptome assembly obtained from liver and testis samples collected from the fifth specimen ever caught of this species.

Results

The deep RNA sequencing performed with Illumina technologies generated 145,435,156 paired-end reads, accounting for ~14 GB of sequence data, which were de novo assembled using a Trinity/CLC combined strategy. The assembly output was processed and filtered producing a set of 66,308 contigs, whose quality was thoroughly assessed. The comparison with the recently

SUBMITTER: Pallavicini A 

PROVIDER: S-EPMC3750513 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature