Three dimensional multiphoton imaging of fresh and whole mount developing mouse mammary glands.

ABSTRACT:

Background

The applications of multiphoton microscopy for deep tissue imaging in basic and clinical research are ever increasing, supplementing confocal imaging of the surface layers of cells in tissue. However, imaging living tissue is made difficult by the light scattering properties of the tissue, and this is extraordinarily apparent in the mouse mammary gland which contains a stroma filled with fat cells surrounding the ductal epithelium. Whole mount mammary glands stained with Carmine Alum are easily archived for later reference and readily viewed using bright field microscopy to observe branching architecture of the ductal network. Here, we report on the advantages of multiphoton imaging of whole mount mammary glands. Chief among them is that optical sectioning of the termina

SUBMITTER: Johnson MD 

PROVIDER: S-EPMC3750743 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature