Functional characterization of three MicroRNAs of the Asian tiger mosquito, Aedes albopictus.

ABSTRACT:

Background

Temporal and stage specific expression of microRNAs (miRNAs) in embryos, larvae, pupae and adults of Aedes albopictus showed differential expression levels across the four developmental stages, indicating their potential regulatory roles in mosquito development. The functional characterization of these miRNAs was not known. Accordingly our study evaluated the functional characterization of three miRNAs, which are temporally up-regulated in the various developmental stages of Ae. albopictus mosquitoes.

Methods

miRNA mimics, inhibitors and negative controls were designed and their knock-in and knock-down efficiency were analyzed by qRT-PCR after transfecting the mosquito cell lines C6/36, and also by injecting in their specific developmental stages. The functional r

SUBMITTER: Puthiyakunnon S 

PROVIDER: S-EPMC3750763 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature