Isolation of midgut escape mutants of two American genotype dengue 2 viruses from Aedes aegypti.

ABSTRACT:

Background

Several studies have shown that American genotype dengue 2 viruses (DENV2) have reduced viral fitness in the mosquito vector, Aedes aegypti, compared to other DENV2 genotypes. Diminished replication efficiency or inability to efficiently traverse membrane barriers encompassing organs such as the midgut or salivary glands are considered major factors negatively impacting viral fitness in the mosquito.

Results

We analyzed the vector competence of Ae. aegypti for two American DENV2 strains, QR94 and PR159 originating from Mexico and Puerto-Rico, respectively. Both strains infected mosquito midguts following acquisition of infectious bloodmeals. However, DENV2-QR94 and DENV2-PR159 poorly disseminated from the midgut at 7 or 14 days post-bloodmeal (pbm). We detected on

SUBMITTER: Khoo CC 

PROVIDER: S-EPMC3751248 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature