Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing.

ABSTRACT:

Background

Penstemon's unique phenotypic diversity, hardiness, and drought-tolerance give it great potential for the xeric landscaping industry. Molecular markers will accelerate the breeding and domestication of drought tolerant Penstemon cultivars by, creating genetic maps, and clarifying of phylogenetic relationships. Our objectives were to identify and validate interspecific molecular markers from four diverse Penstemon species in order to gain specific insights into the Penstemon genome.

Results

We used a 454 pyrosequencing and GR-RSC (genome reduction using restriction site conservation) to identify homologous loci across four Penstemon species (P. cyananthus, P. davidsonii, P. dissectus, and P. fruticosus) representing three diverse subgenera with considerable genome

SUBMITTER: Dockter RB 

PROVIDER: S-EPMC3751293 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature