Aberrant NDRG1 methylation associated with its decreased expression and clinicopathological significance in breast cancer.

ABSTRACT:

Background

Cancer cell differentiation is an important characteristic of malignant tumor and has a great impact on prognosis and therapeutic decision for patients. The N-myc downstream regulated gene 1 (NDRG1), a putative tumor suppression gene, is involved in the regulation of human cell differentiation and metastasis in various cancers. Changes in the status of methylation of the NDRG1 gene have not been studied in detail in human breast cancer.

Results

The MDA-MB-231 breast tumor cell line could express NDRG1. However, it was only expressed after treatment with 5-Aza-2'-deoxycytidine (AZA) in T47D cell line, which revealed that NDRG1 expression could modulated by DNA methylation. Therefore, the fragment surrounding the transcript start site of NDRG1 gene promoter was clon

SUBMITTER: Han LL 

PROVIDER: S-EPMC3751627 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature