On-chip synthesis and screening of a sialoside library yields a high affinity ligand for Siglec-7.

ABSTRACT: The Siglec family of sialic acid-binding proteins are differentially expressed on white blood cells of the immune system and represent an attractive class of targets for cell-directed therapy. Nanoparticles decorated with high-affinity Siglec ligands show promise for delivering cargo to Siglec-bearing cells, but this approach has been limited by a lack of ligands with suitable affinity and selectivity. Building on previous work employing solution-phase sialoside library synthesis and subsequent microarray screening, we herein report a more streamlined 'on-chip' synthetic approach. By printing a small library of alkyne sialosides and subjecting these to 'on-chip' click reactions, the largest sialoside analogue library to date was generated. Siglec-screening identified a selective Siglec-7 l

SUBMITTER: Rillahan CD 

PROVIDER: S-EPMC3751994 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature