TRP-ML1 functions as a lysosomal NAADP-sensitive Ca2+ release channel in coronary arterial myocytes.

ABSTRACT: Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent intracellular Ca(2+) signalling second messenger, but the mechanism of NAADP-induced Ca(2+) release is still poorly understood. The present study tested the hypothesis that NAADP induces Ca(2+) release from the lysosomal store via a TRP-ML1 (transient receptor potential-mucolipin 1)-mediated Ca(2+) release channel in coronary arterial myocytes (CAMs). RT-PCR and Western blot analyses demonstrated that TRP-ML1 was present in CAMs, and fluorescence resonance energy transfer (FRET) detection revealed that the TRP-ML1 was closely associated with some lysosomal proteins in these CAMs. ET-1, a well-known NAADP stimulator, was found to induce a local Ca(2+) burst from lysosomes followed by a global Ca(2+) release. This lysosome-ass

SUBMITTER: Zhang F 

PROVIDER: S-EPMC3752374 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature