A modular strategy for engineering orthogonal chimeric RNA transcription regulators.

ABSTRACT: Antisense RNA transcription attenuators are a key component of the synthetic biology toolbox, with their ability to serve as building blocks for both signal integration logic circuits and transcriptional cascades. However, a central challenge to building more sophisticated RNA genetic circuitry is creating larger families of orthogonal attenuators that function independently of each other. Here, we overcome this challenge by developing a modular strategy to create chimeric fusions between the engineered transcriptional attenuator from plasmid pT181 and natural antisense RNA translational regulators. Using in vivo gene expression assays in Escherichia coli, we demonstrate our ability to create chimeric attenuators by fusing sequences from five different translational regulators. Mutagenesis

SUBMITTER: Takahashi MK 

PROVIDER: S-EPMC3753616 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature