Mapping the LINE1 ORF1 protein interactome reveals associated inhibitors of human retrotransposition.

ABSTRACT: LINE1s occupy 17% of the human genome and are its only active autonomous mobile DNA. L1s are also responsible for genomic insertion of processed pseudogenes and >1 million non-autonomous retrotransposons (Alus and SVAs). These elements have significant effects on gene organization and expression. Despite the importance of retrotransposons for genome evolution, much about their biology remains unknown, including cellular factors involved in the complex processes of retrotransposition and forming and transporting L1 ribonucleoprotein particles. By co-immunoprecipitation of tagged L1 constructs and mass spectrometry, we identified proteins associated with the L1 ORF1 protein and its ribonucleoprotein. These include RNA transport proteins, gene expression regulators, post-translational modifie

SUBMITTER: Goodier JL 

PROVIDER: S-EPMC3753637 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature