Adjustment of trehalose metabolism in wine Saccharomyces cerevisiae strains to modify ethanol yields.

ABSTRACT: The ability of Saccharomyces cerevisiae to efficiently produce high levels of ethanol through glycolysis has been the focus of much scientific and industrial activity. Despite the accumulated knowledge regarding glycolysis, the modification of flux through this pathway to modify ethanol yields has proved difficult. Here, we report on the systematic screening of 66 strains with deletion mutations of genes encoding enzymes involved in central carbohydrate metabolism for altered ethanol yields. Five of these strains showing the most prominent changes in carbon flux were selected for further investigation. The genes were representative of trehalose biosynthesis (TPS1, encoding trehalose-6-phosphate synthase), central glycolysis (TDH3, encoding glyceraldehyde-3-phosphate dehydrogenase), the oxi

SUBMITTER: Rossouw D 

PROVIDER: S-EPMC3753939 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature