Quantification of endospore-forming firmicutes by quantitative PCR with the functional gene spo0A.

ABSTRACT: Bacterial endospores are highly specialized cellular forms that allow endospore-forming Firmicutes (EFF) to tolerate harsh environmental conditions. EFF are considered ubiquitous in natural environments, in particular, those subjected to stress conditions. In addition to natural habitats, EFF are often the cause of contamination problems in anthropogenic environments, such as industrial production plants or hospitals. It is therefore desirable to assess their prevalence in environmental and industrial fields. To this end, a high-sensitivity detection method is still needed. The aim of this study was to develop and evaluate an approach based on quantitative PCR (qPCR). For this, the suitability of functional genes specific for and common to all EFF were evaluated. Seven genes were considere

SUBMITTER: Bueche M 

PROVIDER: S-EPMC3753955 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature