Mode of virus rescue determines the acquisition of VHS mutations in VP22-negative herpes simplex virus 1.

ABSTRACT: It has been proposed that herpes simplex virus 1 with VP22 deleted requires secondary mutation of VHS for viability. Here we show that a replication-competent Δ22 virus constructed by homologous recombination maintains a wild-type (Wt) VHS gene and has no other gross mutations. By contrast, Δ22 viruses recovered from a bacterial artificial chromosome contain multiple amino acid changes within a conserved region of VHS. Hence, the mode of virus rescue influences the acquisition of secondary mutations.

SUBMITTER: Ebert K 

PROVIDER: S-EPMC3753997 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature