The plasma membrane proton pump PMA-1 is incorporated into distal parts of the hyphae independently of the Spitzenkorper in Neurospora crassa.

ABSTRACT: Most models for fungal growth have proposed a directional traffic of secretory vesicles to the hyphal apex, where they temporarily aggregate at the Spitzenkörper before they fuse with the plasma membrane (PM). The PM H(+)-translocating ATPase (PMA-1) is delivered via the classical secretory pathway (endoplasmic reticulum [ER] to Golgi) to the cell surface, where it pumps H(+) out of the cell, generating a large electrochemical gradient that supplies energy to H(+)-coupled nutrient uptake systems. To characterize the traffic and delivery of PMA-1 during hyphal elongation, we have analyzed by laser scanning confocal microscopy (LSCM) strains of Neurospora crassa expressing green fluorescent protein (GFP)-tagged versions of the protein. In conidia, PMA-1-GFP was evenly distributed at the PM.

SUBMITTER: Fajardo-Somera RA 

PROVIDER: S-EPMC3754540 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature