Liquid chromatography/mass spectrometry methods for measuring dipeptide abundance in non-small-cell lung cancer.

ABSTRACT:

Rationale

Metabolomic profiling is a promising methodology of identifying candidate biomarkers for disease detection and monitoring. Although lung cancer is among the leading causes of cancer-related mortality worldwide, the lung tumor metabolome has not been fully characterized.

Methods

We utilized a targeted metabolomic approach to analyze discrete groups of related metabolites. We adopted a dansyl [5-(dimethylamino)-1-naphthalene sulfonamide] derivatization with liquid chromatography/mass spectrometry (LC/MS) to analyze changes of metabolites from paired tumor and normal lung tissues. Identification of dansylated dipeptides was confirmed with synthetic standards. A systematic analysis of retention times was required to reliably identify isobaric dipeptides. We validated o

SUBMITTER: Wu M 

PROVIDER: S-EPMC3755500 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature