Role of connective tissue growth factor in oval cell response during liver regeneration after 2-AAF/PHx in rats.

ABSTRACT:

Background & aims

Recruitment and proliferation of Thy-1+ oval cells is a hallmark of liver regeneration after 2-acetylaminofluorene (2-AAF)/partial hepatectomy (PHx) in rats. To understand the molecular mechanism underlying this process, we investigated the role of connective tissue growth factor (CTGF), one of the candidate genes differentially expressed in Thy-1+ oval cells, in this liver injury model.

Methods

Northern and Western analyses were performed to examine the induction of CTGF in total liver homogenate. Quantitative real-time polymerase chain reaction (PCR), immunofluorescent staining, and in situ hybridization were performed to confirm the expression and localization of CTGF in Thy-1+ oval cells. Finally, a known inhibitor of CTGF synthesis, Iloprost, was admin

SUBMITTER: Pi L 

PROVIDER: S-EPMC3756499 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature