Sulfur amino acids regulate translational capacity and metabolic homeostasis through modulation of tRNA thiolation.

ABSTRACT: Protein translation is an energetically demanding process that must be regulated in response to changes in nutrient availability. Herein, we report that intracellular methionine and cysteine availability directly controls the thiolation status of wobble-uridine (U34) nucleotides present on lysine, glutamine, or glutamate tRNAs to regulate cellular translational capacity and metabolic homeostasis. tRNA thiolation is important for growth under nutritionally challenging environments and required for efficient translation of genes enriched in lysine, glutamine, and glutamate codons, which are enriched in proteins important for translation and growth-specific processes. tRNA thiolation is downregulated during sulfur starvation in order to decrease sulfur consumption and growth, and its absence

SUBMITTER: Laxman S 

PROVIDER: S-EPMC3757545 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature