Ontology highlight
ABSTRACT:
SUBMITTER: Gertow K
PROVIDER: S-EPMC3757745 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Gertow Karin K Hirst Claire E CE Yu Qing C QC Ng Elizabeth S ES Pereira Lloyd A LA Davis Richard P RP Stanley Edouard G EG Elefanty Andrew G AG
Stem cell reports 20130604 1
We investigated the role of canonical WNT signaling in mesoderm and hematopoietic development from human embryonic stem cells (hESCs) using a recombinant human protein-based differentiation medium (APEL). In contrast to prior studies using less defined culture conditions, we found that WNT3A alone was a poor inducer of mesoderm. However, WNT3A synergized with BMP4 to accelerate mesoderm formation, increase embryoid body size, and increase the number of hematopoietic blast colonies. Interestingly ...[more]