Expression and Antigenic Evaluation of VacA Antigenic Fragment of Helicobacter Pylori.

ABSTRACT:

Unlabelled

Objective(s) : Helicobacter pylori, a human specific gastric pathogen is a causative agent of chronic active gastritis. The vacuolating cytotoxin (VacA) is an effective virulence factor involved in gastric injury. The aim of this study was to construct a recombinant protein containing antigenic region of VacA gene and determine its antigenicity.

Materials and methods

The antigenic region of VacA gene was detected by bioinformatics methods. The polymerase chain reaction method was used to amplify a highly antigenic region of VacA gene from chromosomal DNA of H. pylori. The eluted product was cloned into the prokaryotic expression vector pET32a. The target protein was expressed in the Escherichia coli BL21 (DE3) pLysS. The bacteria including pET32a-VacA plasmids wer

SUBMITTER: Hasanzadeh L 

PROVIDER: S-EPMC3758054 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature