Imaging bacterial protein expression using genetically encoded RNA sensors.

ABSTRACT: The difficulties in imaging the dynamics of protein expression in live bacterial cells can be overcome by using fluorescent sensors based on Spinach, an RNA that activates the fluorescence of a small-molecule fluorophore. These RNAs selectively bind target proteins and exhibit fluorescence increases that enable protein expression to be imaged in living Escherichia coli. These sensors are key components of a generalizable strategy to image protein expression in a single bacterium in real time.

SUBMITTER: Song W 

PROVIDER: S-EPMC3758421 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature