Endosomolytic reducible polymeric electrolytes for cytosolic protein delivery.

ABSTRACT: Despite the numerous vital functions of proteins in the cytosolic compartment, less attention has been paid to the delivery of protein drugs to the cytosol than to the plasma membrane. To address this issue and effectively deliver charged proteins into the cytoplasm, we used endosomolytic, thiol-triggered degradable polyelectrolytes as carriers. The cationic, reducible polyelectrolyte RPC-bPEI(0.8 kDa)2 was synthesized by the oxidative polymerization of thiolated branched polyethyleneimine (bPEI). The polymer was converted to the anionic, reducible polyelectrolyte RPA-bPEI(0.8 kDa)2 by introducing carboxylic acids. The two reducible polyelectrolytes (RPC-bPEI(0.8 kDa)2 and RPA-bPEI(0.8 kDa)2) were complexed with counter-charged model proteins (bovine serum albumin (BSA) and lysozyme (LYZ))

SUBMITTER: Tian L 

PROVIDER: S-EPMC3759265 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature