Integrated analysis of global mRNA and protein expression data in HEK293 cells overexpressing PRL-1.

ABSTRACT:

Background

The protein tyrosine phosphatase PRL-1 represents a putative oncogene with wide-ranging cellular effects. Overexpression of PRL-1 can promote cell proliferation, survival, migration, invasion, and metastasis, but the underlying mechanisms by which it influences these processes remain poorly understood.

Methodology

To increase our comprehension of PRL-1 mediated signaling events, we employed transcriptional profiling (DNA microarray) and proteomics (mass spectrometry) to perform a thorough characterization of the global molecular changes in gene expression that occur in response to stable PRL-1 overexpression in a relevant model system (HEK293).

Principal findings

Overexpression of PRL-1 led to several significant changes in the mRNA and protein expression p

SUBMITTER: Dumaual CM 

PROVIDER: S-EPMC3760866 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature