Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

ABSTRACT: We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

SUBMITTER: Yu Z 

PROVIDER: S-EPMC3761309 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature