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Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

ABSTRACT: We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

SUBMITTER: Yu Z 

PROVIDER: S-EPMC3761309 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

Yu Zhongsheng Z   Ren Mengda M   Wang Zhanxiang Z   Zhang Bo B   Rong Yikang S YS   Jiao Renjie R   Gao Guanjun G  

Genetics 20130705 1

We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila. ...[more]

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