Interlaboratory reproducibility of selective reaction monitoring assays using multiple upfront analyte enrichment strategies.

ABSTRACT: Over the past few years, mass spectrometry has emerged as a technology to complement and potentially replace standard immunoassays in routine clinical core laboratories. Application of mass spectrometry to protein and peptide measurement can provide advantages including high sensitivity, the ability to multiplex analytes, and high specificity at the amino acid sequence level. In our previous study, we demonstrated excellent reproducibility of mass spectrometry-selective reaction monitoring (MS-SRM) assays when applying standardized standard operating procedures (SOPs) to measure synthetic peptides in a complex sample, as lack of reproducibility has been a frequent criticism leveled at the use of mass spectrometers in the clinical laboratory compared to immunoassays. Furthermore, an importa

SUBMITTER: Prakash A 

PROVIDER: S-EPMC3761372 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature