Glycerol modulates water permeation through Escherichia coli aquaglyceroporin GlpF.

ABSTRACT: Among aquaglyceroporins that transport both water and glycerol across the cell membrane, Escherichia coli glycerol uptake facilitator (GlpF) is the most thoroughly studied. However, one question remains: Does glycerol modulate water permeation? This study answers this fundamental question by determining the three-dimensional potential of mean force of glycerol along the permeation path through GlpF's conducting pore. There is a deep well near the Asn-Pro-Ala (NPA) motifs (6.5kcal/mol below the bulk level) and a barrier near the selectivity filter (10.1kcal/mol above the well bottom). This profile owes its existence to GlpF's perfect steric arrangement: The glycerol-protein van der Waals interactions are attractive near the NPA but repulsive elsewhere in the conducting pore. In light of the

SUBMITTER: Chen LY 

PROVIDER: S-EPMC3761968 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature