Dual dependence of cryobiogical properties of Sf21 cell membrane on the temperature and the concentration of the cryoprotectant.

ABSTRACT: The Sf21 cell line is extensively used for virus research and producing heterologous recombinant proteins. To develop optimal strategies for minimizing cell injury due to intracellular ice formation and excessive volume shrinkage during cryopreservation, the fundamental transport properties including the osmotic inactive volume (Vb ), the hydraulic conductivity (Lp ), and the glycerol permeability (Ps ) of Sf21 cell membrane at 25, 15, 5 and -2°C were characterized using a micro-perfusion chamber. The effects of temperature on the hydraulic conductivity and the glycerol permeability of Sf21 cell membrane, reflected by the activation energies, were quantitatively investigated. It was found that the hydraulic conductivity decreases along with the increase of the final CPA concentration at a

SUBMITTER: Wang J 

PROVIDER: S-EPMC3762842 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature