Reversible protein affinity-labelling using bromomaleimide-based reagents.

ABSTRACT: Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild conditions of using a stoichiometric amount of a bis-thiol, thus providing an approach that will be of general interest in chemical biology and proteomics.

SUBMITTER: Nathani RI 

PROVIDER: S-EPMC3763775 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature