Microfluidic system for measuring neutrophil migratory responses to fast switches of chemical gradients.

ABSTRACT: Experimental systems that provide temporal and spatial control of chemical gradients are required for probing into the complex mechanisms of eukaryotic cell chemotaxis. However, no current technique can simultaneously generate stable chemical gradients and allow fast gradient changes. We developed a microfluidic system with microstructured membranes for exposing neutrophils to fast and precise changes between stable, linear gradients of the known chemoattractant Interleukin-8 (IL-8). We observed that rapidly lowering the average concentration of IL-8 within a gradient, while preserving the direction of the gradient, resulted in temporary neutrophil depolarization. Fast reversal of the gradient direction while increasing or decreasing the average concentration also resulted in temporary dep

SUBMITTER: Irimia D 

PROVIDER: S-EPMC3763904 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature