Semi-automated curation of metabolic models via flux balance analysis: a case study with Mycoplasma gallisepticum.

ABSTRACT: Primarily used for metabolic engineering and synthetic biology, genome-scale metabolic modeling shows tremendous potential as a tool for fundamental research and curation of metabolism. Through a novel integration of flux balance analysis and genetic algorithms, a strategy to curate metabolic networks and facilitate identification of metabolic pathways that may not be directly inferable solely from genome annotation was developed. Specifically, metabolites involved in unknown reactions can be determined, and potentially erroneous pathways can be identified. The procedure developed allows for new fundamental insight into metabolism, as well as acting as a semi-automated curation methodology for genome-scale metabolic modeling. To validate the methodology, a genome-scale metabolic model for

SUBMITTER: Bautista EJ 

PROVIDER: S-EPMC3764002 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature